Spatial distribution characteristics of carbazole and polyhalogenated carbazoles in water column and sediments in the open Western Pacific Ocean.

Polyhalogenated carbazoles (PHCZs), an emerging persistent halogenated organic pollutant, have been detected in the environment. However, our understanding of PHCZs in the ocean remains limited. In this study, 47 seawater samples (covering 50 - 4000 m) and sediment samples (49 surface and 3 cores) were collected to investigate the occurrence and spatial distribution patterns of carbazole and its halogenated derivants (CZDs) in the Western Pacific Ocean. In seawater, the detection frequencies of CZ (97.87%) and 3-CCZ (57.45%) were relatively high. In addition, the average concentration of ΣPHCZs in the upper water (< 150 m, 0.23 ± 0.21 ng/L) was significantly lower than that in the deep ocean (1000 - 4000 m, 0.65 ± 0.56 ng/L, P < 0.05), which may indicate the vertical transport of PHCZs in the marine environment. The concentration of ΣCZDs in surface sediment ranges from 0.46 to 6.48 ng/g (mean 1.54 ng/g), among which CZ and 36-CCZ were the predominant components. Results from sediment cores demonstrate a noteworthy negative correlation between the concentration of CZDs and depth, indicating the ongoing natural degradation process occurring in sediment cores over a long period. This study offers distinctive insights into the occurrence, composition, and vertical features of CZDs in oceanic environments.

Voyaging of halogenated polycyclic aromatic hydrocarbons, an emerging group of pollutants, on micro-mesoplastics in the marine environment.

The limited existing research on the accumulation of hazardous chlorinated and brominated polycyclic aromatic hydrocarbons (ClPAHs and BrPAHs) in micro-mesoplastics (mMPs) motivated this investigation. We collected mMPs from the coastal environments of Sri Lanka and Japan. Out of 75 target compounds analyzed, 61 were detected, with total parent PAH concentrations reaching 16,300 and 1770 ng/g plastic in Sri Lanka and Japan, respectively. The total parent PAH concentrations in mMPs from the southern Sri Lankan coastline were relatively higher than those from the eastern coastline. Phenanthrene and naphthalene were the dominant parent PAH congeners in most mMP samples. Chlorinated pyrenes and brominated naphthalene were predominant among halogenated PAHs. The estimated toxic equivalency quotient (TEQ) ranged from 0.67 to 1057 ng-TEQ/g plastic, with the highest levels observed in polystyrene (PS) particles from the southern Sri Lankan coast. Benzo[a]pyrene and dibenzo[a,h]anthracene exhibited elevated TEQ for parent PAHs, whereas dichloropyrene, and dibromopyrene represented the highest TEQs for ClPAHs and BrPAHs, respectively. The data evidenced that several HPAH congeners can increase the PAH-like toxicity (∼86%) in mMPs. This study provides insights into the accumulation of parent and halogenated PAHs in mMPs, highlighting their potential combined implications in marine and terrestrial ecosystems.

Comparative Study on the Distribution of Essential, Non-Essential Toxic, and Other Elements across Trophic Levels in Various Edible Aquatic Organisms in Sri Lanka and Dietary Human Risk Assessment.

Thirty-six elements are categorized as essential but toxic in excess amount (EBTEs), non-essential toxic (NETs), and Other in 29 different edible aquatic species dwelling in offshore pelagic, and coastal and estuarine (CE) ecosystems were investigated in Sri Lanka. Elements were analyzed using an energy-dispersive X-ray fluorescence (EDXRF) spectrometer, and an NIC MA-3000 Mercury Analyzer. EBTEs showed a negative relationship, whereas NETs showed a positive relationship between the concentration (mg/kg wet weight) and trophic levels in both ecosystems. EBTEs showed trophic dilution, whereas NETs showed trophic magnification. Some elements in a few organisms exceeded the maximum allowable limit which is safe for human consumption. There was a positive relationship (R2 = 0.85) between the concentration of mercury and body weight of yellowfin tuna (YFT). For the widely consumed YFT, the calculated hazard index (HI) for the non-carcinogenic health and exposure daily intake of NETs for adults were 0.27 and 9.38 × 10-5 mg/kg bw/day, respectively. The estimated provisional tolerable weekly intake (PTWI) (µg/kg bw/w) was 0.47 for arsenic and 0.05 for antimony, cadmium, mercury, and lead. The HI and PTWI values were below the recommended limits; thus, consumption of YFT does not pose any health risk for Sri Lankan adults.

Presence of Halogenated Polycyclic Aromatic Hydrocarbons in Milk Powder and the Consequence to Human Health.

Recent reports of the presence of halogenated derivatives of polycyclic aromatic hydrocarbons (PAHs) in human foods of animal origin, such as chlorinated (ClPAHs) and brominated (BrPAHs) PAHs, suggest that their contamination in dairy products may also pose a human health risk. This study used GC/Orbitrap-MS to analyze 75 congeners of halogenated PAHs and parent PAHs in milk and creaming powder samples commonly found in grocery stores in Sri Lanka and Japan. Our investigation revealed a total of 31 halogenated PAHs (HPAHs) in the samples. The concentrations of total parent PAHs in the samples from Sri Lanka and Japan ranged from not detected (n.d.)−0.13 and <0.001−16 ng/g dry weight (d.w.). Total ClPAHs and BrPAHs in the samples ranged from 0.01−3.35 and 1.20−5.15 ng/g (d.w.) for Sri Lanka, and 0.04−2.54 and n.d.−2.03 ng/g d.w. for Japan, respectively. The ClPAHs were dominated by chlorinated-pyrene, -fluoranthene, and -benzo[a]pyrene congeners, whereas the BrPAHs were dominated by brominated-naphthalene and -pyrene congeners. The toxic assessment estimated based on the intake of toxic equivalency quotients (TEQs) for target compounds in milk powders revealed that HPAHs might contribute additively to the PAHs-associated health risk to humans, indicating that more research is needed.

Halogenated polycyclic aromatic hydrocarbons in edible aquatic species of two Asian countries: Congener profiles, biomagnification, and human risk assessment.

Seventy-five contaminants including chlorinated/brominated/parent polycyclic aromatic hydrocarbons (Cl/Br/PAHs) were investigated in 29 edible aquatic species from the Indian Ocean near Sri Lanka and 10 species from the Pacific Ocean near Japan. Concentrations of total ClPAHs and BrPAHs in the samples were 2.6-57 and 0.30-9.5 ng/g-dry weight from the Indian Ocean, and 0.35-18 and 0.03-3.3 ng/g-dry weight from the Pacific Ocean, respectively. Comparing the profiles of Cl/BrPAHs among the samples, congeners of chlorinated and brominated pyrene were predominant components and enhanced the potential for biomagnification in the sample from the off-shore pelagic environment in the Indian Ocean. The incremental lifetime cancer risks estimated by intake of the targets in consuming aquatic organisms showed that approximately one-third of studied organisms exceeded the acceptable risk level for Sri Lankans.